Can a plan achromat objective be used for fluorescence with reflected light?

In principle, yes. Plan achromat objectives can deliver acceptable results with blue or green excitation wavelengths.

• The glass elements may exhibit autofluorescence under near-UV excitation, which can interfere with the signal
• A lower numerical aperture (NA) compared to plan fluorite or plan apochromat objectives may result in reduced image brightness
• Not ideal for critical fluorescence applications requiring maximum brightness and precise color correction

• Use plan achromats only for basic fluorescence with shorter wavelengths (blue/green)
• For bright, high-contrast fluorescence imaging, plan fluorite or plan apochromat objectives are better suited

Do you work with fluorescence and want to select the right objectives?